The TFAM Polyclonal Antibody (CAB1926) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly reactive with human samples, making it a reliable choice for various research applications.TFAM, also known as mitochondrial transcription factor A, plays a pivotal role in mitochondrial biogenesis and function by binding to mitochondrial DNA and regulating transcription. Its function is crucial for cellular energy production and metabolism, making it a key player in various physiological processes.The TFAM Polyclonal Antibody (CAB1926) enables the detection and analysis of TFAM in different cell types, providing valuable insights into mitochondrial function and dysfunction.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TFAM Polyclonal Antibody
SKU:
CAB1926
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7019
RRID
AB_2763953
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TFAM Rabbit pAb (CAB1926) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using TFAM Rabbit pAb (CAB1926). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
