The Tyrosine Hydroxylase Antibody (CAB12756) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. RRID AB_2759601 Gene ID 7054 Swiss Prot Synonym TYH; DYT14; DYT5b; Tyrosine Hydroxylase
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Tyrosine Hydroxylase Antibody
SKU:
CAB12756
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:50 - 1:200
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TYH, DYT14, DYT5b, Tyrosine Hydroxylase
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Axon, Cytoplasm, Cytoplasmic Side Of Plasma Membrane, Cytoplasmic Vesicle, Cytosol, Dendrite, Mitochondrion, Neuron Projection, Nucleus, Perikaryon, Perinuclear Region Of Cytoplasm, Smooth Endoplasmic Reticulum, Synaptic Vesicle, Terminal Bouton.
Calculated MW:
45-59 kDa
Observed MW:
60 kDa
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. RRID AB_2759601 Gene ID 7054 Swiss Prot Synonym TYH; DYT14; DYT5b; Tyrosine Hydroxylase
Purification Method:
Affinity purification
Gene ID:
7054
RRID:
AB_2759601
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Tyrosine Hydroxylase Rabbit pAb (CAB12756) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat adrenal gland using Tyrosine Hydroxylase Rabbit pAb (CAB12756) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using Tyrosine Hydroxylase Rabbit pAb(CAB12756) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Tyrosine Hydroxylase Rabbit pAb(CAB12756) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
