Description
TIAM1 Antibody (CAB10252)
The TIAM1 Antibody (CAB10252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the TIAM1 protein, researchers can accurately detect and analyze its expression in various cell types, making it an ideal choice for studies in cancer research and cell biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
| Product Name: | TIAM1 Antibody |
| SKU: | CAB10252 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | SILR DKHR RQLL KTES LPSS QQYV PFGG KRLC ALKG ARPA MSRA VSAP SKSL GRRR RRLA RNRF TIDS DAVS ASSP EKES QQPP GGGD TDRW VEEQ FDLA QYEE QDDI KETD ILSD DDEF CESV KGAS VDRD LQER LQAT SISQ RERG RKTL DSHA SRMA QLKK QAAL SGIN GGLE SASE EVIW VRRE DFAP SRKL NTEI | ||||||
| Tested Applications: | WB IHC-P ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | NEDLDS, TIAM-1, TIAM1 |
| Positive Sample: | Rat testis |
| Cellular Localization: | Cell Junction, Cell Membrane, Cytoplasmic Side, Peripheral Membrane Protein. |
| Calculated MW: | 178kDa |
| Observed MW: | 250kDa |
This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain.
| Purification Method | Affinity purification |
| Gene ID | 7074 |
| RRID | AB_2757779 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from Rat testis, using TIAM1 Rabbit pAb (CAB10252) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TIAM1 Rabbit pAb (CAB10252) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
