The TIFAB Monoclonal Antibody (CAB24024) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed specifically for use in human samples, is highly specific and validated for use in various applications such as Western blot and immunofluorescence. By targeting the TIFAB protein, this antibody enables precise detection and analysis in different cell types, making it essential for studies in hematology, immunology, and cancer research.TIFAB, or TIFA-related adapter protein, plays a vital role in regulating the immune response and inflammatory pathways, making it a potential target for therapeutic interventions in diseases such as leukemia, lymphoma, and other hematologic malignancies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TIFAB Monoclonal Antibody
SKU:
CAB24024
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC62261
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293T transfected with TIFAB（Human）, MV-4-11
Cellular Localization:
Cytoplasm.
Calculated MW:
18KDa
Observed MW:
18kDa
TIFAB binds to TIFA (MIM 609028) and inhibits TIFA mediated NF kappa-B (NFKB1; MIM 164011) activation.
Purification Method
Affinity purification
Gene ID
497189
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TIFAB using [KD Validated] TIFAB Rabbit mAb (CAB24024) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Western blot analysis of lysates from wild type (WT) and TIFAB knockdown (KD) MV-4-11 cells using [KD Validated] TIFAB Rabbit mAb (CAB24024) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
