The Timeless Monoclonal Antibody (CAB0512) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding to the Timeless protein, this antibody enables precise detection and analysis in different cell types, making it an ideal choice for studies in genetics, cell biology, and chronobiology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Timeless Monoclonal Antibody
SKU:
CAB0512
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1827
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIM, TIM1, hTIM, FASPS4, Timeless
Positive Sample:
293T, Hep G2, A-549
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
139kDa
Observed MW:
150kDa
The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders.
Purification Method
Affinity purification
Gene ID
8914
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Timeless Rabbit mAb (CAB0512) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
