The TIMM22 Monoclonal Antibody (CAB23557) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, demonstrates high specificity and sensitivity for detecting TIMM22 in various cell types and tissues, making it ideal for studies in mitochondrial biology and cellular metabolism.TIMM22 plays a pivotal role in the transport of proteins across the inner mitochondrial membrane, contributing to the maintenance of mitochondrial function and cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIMM22 Monoclonal Antibody
SKU:
CAB23557
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3118
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TEX4, TIM22, COXPD43, TIMM22
Positive Sample:
Hep G2, Mouse ovary, Rat heart
Calculated MW:
20kDa
Observed MW:
20kDa
Multipass transmembrane proteins are brought into mitochondria and inserted into the mitochondrial inner membrane by way of the TIM22 complex. This complex has six subunits and is a twin-pore translocase. The protein encoded by this gene is a subunit of TIM22 and represents the voltage-activated and signal-gated channel.
Purification Method
Affinity purification
Gene ID
29928
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells, using TIMM22 Rabbit mAb (CAB23557) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates, using TIMM22 Rabbit mAb (CAB23557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
