The TIMM8B Antibody (CAB15814) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is optimized for use in various experimental applications, including Western blotting and immunofluorescence microscopy. It binds specifically to TIMM8B, facilitating the detection and analysis of this important mitochondrial protein in different cell types.TIMM8B is a crucial component of the mitochondrial protein translocase complex, playing a key role in the import of nuclear-encoded proteins into the mitochondria. Dysregulation of TIMM8B has been linked to various mitochondrial disorders and neurodegenerative diseases.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIMM8B Antibody
SKU:
CAB15814
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.
Purification Method
Affinity purification
Gene ID
26521
RRID
AB_2763237
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TIMM8B Rabbit pAb (CAB15814) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using TIMM8B Rabbit pAb (CAB15814) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using TIMM8B Rabbit pAb (CAB15814) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using TIMM8B Rabbit pAb (CAB15814) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
