The TLE1 Monoclonal Antibody (CAB3528) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, is highly specific and sensitive for detecting TLE1 in human samples, making it ideal for use in immunohistochemistry and immunofluorescence applications.TLE1 is known for its role in repressing gene transcription by interacting with various transcription factors, such as TCF/LEF and Notch.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TLE1 Monoclonal Antibody
SKU:
CAB3528
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0793
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ESG, ESG1, GRG1, TLE-1, TLE1
Positive Sample:
U-87 MG, Mouse lung, Rat lung
Cellular Localization:
Nucleus.
Calculated MW:
83kDa
Observed MW:
83kDa
Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex.
Purification Method
Affinity purification
Gene ID
7088
RRID
AB_2863078
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TLE1 Rabbit mAb (CAB3528) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of various lysates using TLE1 Rabbit mAb (CAB3528) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
