The TMEM176B Polyclonal Antibody (CAB24509) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the TMEM176B protein, this antibody enables precise detection and analysis in a variety of cell types, making it perfect for investigations in immunology and cancer research.The TMEM176B protein is known to be involved in lysosomal function and regulation of immune responses, suggesting its importance in various disease contexts, including cancer and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TMEM176B Polyclonal Antibody
SKU:
CAB24509
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TMEM176B, LR8, MS4B2, transmembrane protein 176B
Positive Sample:
A549, OVCAR-3, THP-1, Mouse kidney, Mouse lung
Cellular Localization:
Multi-Pass Membrane Protein, Nucleus Membrane.
Calculated MW:
25kDa/29kDa
Observed MW:
25kDa
TMEM176B (transmembrane protein 176B) is a protein-coding gene. An important paralog of this gene is TMEM176A.
Purification Method
Affinity purification
Gene ID
28959
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TMEM176B Rabbit pAb (CAB24509) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of various lysates, using TMEM176B Rabbit pAb (CAB24509) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
