Anti-GITR/TNFRSF18 Antibody (CAB15625)

Antibody Name:	Anti-GITR/TNFRSF18 Antibody
Antibody SKU:	CAB15625
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-255 of human GITR/TNFRSF18 (NP_683699.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-255 of human GITR/TNFRSF18 (NP_683699.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QRPT GGPG CGPG RLLL GTGT DARC CRVH TTRC CRDY PGEE CCSE WDCM CVQP EFHC GDPC CTTC RHHP CPPG QGVQ SQGK FSFG FQCI DCAS GTFS GGHE GHCK PWTD CCWR CRRR PKTP EAAS SPRK SGAS DRQR RRGG WETC GCEP GRPP GPPT AASP SPGA PQAA GALR SALG RALL PWQQ KWVQ EGGS DQRP GPCS SAAA AGPC RRER ETQS WPPS SLAG PDGV GS
Gene ID:	8784
Uniprot:	Q9Y5U5
Cellular Location:
Calculated MW:	25kDa/26kDa
Observed MW:	30kDa

Synonyms:

TNFRSF18, AITR, CD357, GITR, GITR-D

Background:

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

UniProt Protein Function:	TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, cytokine; Apoptosis Chromosomal Location of Human Ortholog: 1p36.3 Cellular Component: external side of plasma membrane; extracellular region; integral to plasma membrane; plasma membrane Molecular Function:tumor necrosis factor receptor activity Biological Process: immune response; induction of apoptosis via death domain receptors; inflammatory response; multicellular organismal development; negative regulation of apoptosis; positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of cell proliferation; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway
NCBI Summary:	This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]
UniProt Code:	Q9Y5U5
NCBI GenInfo Identifier:	13878830
NCBI Gene ID:	8784
NCBI Accession:	Q9Y5U5.1
UniProt Secondary Accession:	Q9Y5U5,O95851, Q5U0I4, Q9NYJ9, B1AME1,
UniProt Related Accession:	Q9Y5U5
Molecular Weight:	25,110 Da
NCBI Full Name:	Tumor necrosis factor receptor superfamily member 18
NCBI Synonym Full Names:	tumor necrosis factor receptor superfamily member 18
NCBI Official Symbol:	TNFRSF18
NCBI Official Synonym Symbols:	AITR; GITR; CD357; GITR-D
NCBI Protein Information:	tumor necrosis factor receptor superfamily member 18
UniProt Protein Name:	Tumor necrosis factor receptor superfamily member 18
UniProt Synonym Protein Names:	Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD_antigen: CD357
UniProt Gene Name:	TNFRSF18
UniProt Entry Name:	TNR18_HUMAN

Western blot analysis of extracts of various cell lines, using GITR/TNFRSF18 antibody (CAB15625) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)