The 4-1BBL/CD137L Monoclonal Antibody (CAB3386) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is raised in rabbits and is highly reactive with human samples, making it suitable for use in Western blot applications for the detection and analysis of the CD137L protein in various cell types.The CD137L protein, also known as tumor necrosis factor ligand superfamily member 9 (TNFSF9), plays a crucial role in immune responses, particularly in the regulation of T cell activation and proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
4-1BBL/CD137L Monoclonal Antibody
SKU:
CAB3386
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1963
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD137L, TNLG5A, 4-1BB-L, 4-1BBL/CD137L
Positive Sample:
HeLa, Raji, SH-SY5Y, Mouse lung, Mouse brain, Rat lung
Cellular Localization:
Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
27kDa
Observed MW:
27kDa
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Purification Method
Affinity purification
Gene ID
8744
RRID
AB_2863048
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using 4-1BBL/CD137L Rabbit mAb (CAB3386) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
