The TNK1 Polyclonal Antibody (CAB21300) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications, enabling researchers to detect and analyze TNK1 protein levels in various cell types.TNK1, also known as tyrosine kinase non-receptor 1, is involved in regulating cell growth, differentiation, and survival, making it a target of interest in cancer research. Its overexpression has been linked to tumor progression and metastasis, highlighting its potential as a therapeutic target for cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TNK1 Polyclonal Antibody
SKU:
CAB21300
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KOS1, TNK1
Positive Sample:
T-47D
Cellular Localization:
Cytoplasm.
Calculated MW:
72kDa
Observed MW:
72kDa
The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8711
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from T-47D cells, using TNK1 Rabbit pAb (CAB21300) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
