The TOPBP1 Polyclonal Antibody (CAB5781) is a valuable tool for researchers studying TOPBP1, a protein involved in DNA damage response and cell cycle regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the TOPBP1 protein, this antibody allows for the detection and analysis of TOPBP1 levels in various cell types, making it ideal for studies in molecular biology and cancer research.TOPBP1, also known as DNA topoisomerase II binding protein 1, is a key player in DNA repair pathways and cell cycle checkpoint control.
Dysregulation of TOPBP1 has been implicated in cancer development and progression, making it a promising target for therapeutic interventions. Research into the function and regulation of TOPBP1 is essential for understanding its role in genome stability and potential implications for cancer treatment strategies.Overall, the TOPBP1 Polyclonal Antibody (CAB5781) provides researchers with a reliable tool for investigating the role of TOPBP1 in various biological processes, paving the way for new insights into cancer biology and potential therapeutic targets.
Product Name:
TOPBP1 Rabbit Polyclonal Antibody
SKU:
CAB5781
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
171kDa
Observed MW:
200kDa
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Purification Method:
Affinity purification
Gene ID:
11073
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HCT116 cells, using TOPBP1 Rabbit pAb (CAB5781) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
