The TP53BP2 Polyclonal Antibody (CAB21633) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications. By binding specifically to the TP53BP2 protein, this antibody enables the efficient detection and analysis of TP53BP2 levels in various cell types.TP53BP2, also known as tumor protein p53 binding protein 2, plays a crucial role in mediating the cellular response to DNA damage, thereby safeguarding genomic integrity and preventing tumorigenesis. Dysregulation of TP53BP2 has been linked to various human diseases, including cancer, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TP53BP2 Polyclonal Antibody
SKU:
CAB21633
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBP, 53BP2, ASPP2, P53BP2, PPP1R13A, TP53BP2
Positive Sample:
DU 145, OVCAR-3, K-562, A549
Cellular Localization:
Cytoplasm, Nucleus, Perinuclear Region.
Calculated MW:
126kDa
Observed MW:
Refertofigures
This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7159
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TP53BP2 Rabbit pAb (CAB21633) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Jurkat cells using TP53BP2 Rabbit pAb (CAB21633) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
