The TPK1 Antibody (CAB4634) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the TPK1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for studies in biochemistry and metabolism research.TPK1 is a crucial enzyme in the thiamine pyrophosphate synthesis pathway, playing a vital role in cellular energy production and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TPK1 Antibody
SKU:
CAB4634
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP20, HTPK1, THMD5, TPK1
Positive Sample:
Mouse kidney, Rat kidney
Cellular Localization:
Cytosol.
Calculated MW:
27kDa
Observed MW:
27kDa
The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5.
Purification Method
Affinity purification
Gene ID
27010
RRID
AB_2765797
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TPK1 Rabbit pAb (CAB4634) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
