The TRAF4 Monoclonal Antibody (CAB22472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using advanced monoclonal antibody technology, offers high specificity and sensitivity for detecting TRAF4 in various cell types and tissues.TRAF4, a member of the TRAF family of proteins, serves as a crucial mediator in immune responses, particularly in the regulation of cytokine production and cell survival. Dysregulation of TRAF4 has been implicated in various diseases, including cancer, inflammation, and autoimmune disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRAF4 Monoclonal Antibody
SKU:
CAB22472
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57454
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined.
Purification Method
Affinity purification
Gene ID
9618
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat thymus using TRAF4 Rabbit mAb (CAB22472) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Western blot analysis of lysates from MCF7 cells using TRAF4 Rabbit mAb (CAB22472) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
