The TRIM21 Antibody (CAB1957) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is ideal for use in Western blot applications. By targeting the Trim21 protein, this antibody allows for precise detection and analysis of Trim21 expression in a variety of cell types, making it a useful tool in immunology and cancer research studies.Trim21, also known as Ro52, is a key player in the immune system, functioning as an E3 ubiquitin ligase and contributing to the regulation of inflammatory responses.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM21 Antibody
SKU:
CAB1957
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
Purification Method
Affinity purification
Gene ID
6737
RRID
AB_2763983
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung using TRIM21/SS-A Rabbit pAb (CAB1957) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:180s.
Western blot analysis of lysates from wild type (WT) and TRIM21/SS-A knockout (KO) 293T cells using TRIM21/SS-A Rabbit pAb (CAB1957) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of NIH/3T3 cells using TRIM21/SS-A Rabbit pAb (CAB1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using TRIM21/SS-A Rabbit pAb (CAB1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of TRIM21/SS-A in 200 µg extracts from Hep G2 cells using 0.5 µg TRIM21/SS-A Rabbit pAb (CAB1957). Western blot analysis was performed using TRIM21/SS-A Rabbit pAb (CAB1957) at 1:1500 dilution.
