The TRIM25 Monoclonal Antibody (CAB4347) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, demonstrates high specificity and sensitivity for TRIM25 in various experimental settings.TRIM25 is a crucial regulator of the innate immune response, particularly in the detection and signaling of viral infections. By targeting TRIM25, researchers can investigate its role in antiviral defense mechanisms and its potential as a therapeutic target in viral diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TRIM25 Monoclonal Antibody
SKU:
CAB4347
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0971
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EFP, Z147, RNF147, ZNF147, 25
Positive Sample:
293T
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
70kDa
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Purification Method
Affinity purification
Gene ID
7706
RRID
AB_2863242
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb (CAB4347) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
