The TRIM5 Antibody (CAB17234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to TRIM5 and is validated for use in various applications such as Western blot and immunofluorescence.TRIM5, a tripartite motif-containing protein, acts as a restriction factor against retroviral infections by targeting the viral capsid. It has been implicated in the host defense mechanism against HIV and other retroviruses, making it a key target for investigation in virology and immunology research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM5 Antibody
SKU:
CAB17234
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RNF88, TRIM5alpha, TRIM5
Positive Sample:
HeLa, SGC-7901, Mouse stomach
Cellular Localization:
Cytoplasm, Nucleus, P-Body.
Calculated MW:
56kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
85363
RRID
AB_2772714
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRIM5 Rabbit pAb (CAB17234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
