The TRIM62 Antibody (CAB15855) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications. By specifically binding to the TRIM62 protein, this antibody enables precise detection and analysis in various cell types, making it an invaluable asset for studies in immunology, virology, and infectious disease research.TRIM62, a member of the tripartite motif (TRIM) protein family, plays a crucial role in modulating immune signaling pathways and promoting the antiviral response.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM62 Antibody
SKU:
CAB15855
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
HEQH QVTG IDDA FDEL QREL KDQL QALQ DSER EHTE ALQL LKRQ LAET KSST KSLR TTIG EAFE RLHR LLRE RQKA MLEE LEAD TART LTDI EQKV QRYS QQLR KVQE GAQI LQER LAET DRHT FLAG VASL SERL KGKI HETN LTYE DFPT SKYT GPLQ Y
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DEAR1, TRIM62
Positive Sample:
A-431, Mouse brain
Cellular Localization:
Cytoplasm.
Calculated MW:
54kDa
Observed MW:
54kDa
Enables identical protein binding activity; transcription coactivator activity; and ubiquitin-protein transferase activity. Involved in several processes, including negative regulation of viral transcription; positive regulation of NF-kappaB transcription factor activity; and positive regulation of antifungal innate immune response. Is active in cytoplasm.
Purification Method
Affinity purification
Gene ID
55223
RRID
AB_2763282
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRIM62 Rabbit pAb (CAB15855) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using TRIM62 Rabbit pAb (CAB15855) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
