The TRIM66 Antibody (CAB18411) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it an excellent choice for Western blot applications. By binding to Trim66, the antibody enables the detection and analysis of this protein in various cell types, allowing for in-depth studies in the fields of epigenetics, gene regulation, and cancer research.Trim66, a member of the tripartite motif (TRIM) protein family, is involved in diverse cellular processes, including transcriptional regulation and protein degradation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TRIM66 Antibody
SKU:
CAB18411
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIF1D, C11orf29, TIF1DELTA, TRIM66
Positive Sample:
SGC-7901, 293T
Cellular Localization:
Nucleoplasm.
Calculated MW:
149kDa
Observed MW:
135kDa
Predicted to enable chromatin binding activity and identical protein binding activity. Predicted to act upstream of or within negative regulation of transcription, DNA-templated. Located in aggresome and nucleoplasm.
Purification Method
Affinity purification
Gene ID
9866
RRID
AB_2862178
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRIM66 Rabbit pAb (CAB18411) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
