The TriMethyl-Histone H3-K79 Antibody (CAB2369) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets the trimethylated form of histone H3 at lysine 79, a modification associated with transcriptional regulation and DNA repair processes.Histone modifications play a key role in regulating gene expression by altering the chromatin structure, thereby influencing DNA accessibility for transcription factors. The trimethylation of histone H3 at lysine 79 is linked to various cellular processes, including DNA damage response and cell cycle regulation.With its high specificity and sensitivity, the Trimethyl Histone H3 (K79) Polyclonal Antibody enables researchers to detect and analyze this specific histone modification in various cell types and experimental conditions.
This antibody is validated for use in WB, IHC-P, ChIP, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
TriMethyl-Histone H3-K79 Antibody
SKU:
CAB2369
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DFKT D
Tested Applications:
WBIHC-PChIPELISADB
Recommended Dilution:
WB
1:500 - 1:1000
DB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2736929
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using TriMethyl-Histone H3-K79 Rabbit pAb (CAB2369) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 300s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (CAB2369) at 1:1000 dilution.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K79 antibody (CAB2369) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
