The TRIP12 Antibody (CAB9958) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting experiments. By specifically binding to TRIP12, researchers can effectively detect and analyze the protein in a wide range of cell types, making it ideal for investigations in areas such as molecular biology and cancer research.TRIP12, also known as thyroid hormone receptor interactor 12, plays a crucial role in maintaining cellular homeostasis by regulating the turnover of proteins and repairing damaged DNA.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIP12 Antibody
SKU:
CAB9958
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ULF, MRD49, TRIPC, TRIP-12, TRIP12
Positive Sample:
HeLa, Jurkat
Cellular Localization:
Nucleus, Nucleoplasm.
Calculated MW:
220kDa
Observed MW:
260kDa
The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53.
Purification Method
Affinity purification
Gene ID
9320
RRID
AB_2772719
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TRIP12 Rabbit pAb (CAB9958) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon using TRIP12 Rabbit pAb (CAB9958) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using TRIP12 Rabbit pAb (CAB9958) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
