The TSEN54 Antibody (CAB15227) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2. RRID AB_2762121 Gene ID 283989 Swiss Prot Synonym PCH4; PCH5; PCH2A; sen54; SEN54L; TSEN54
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TSEN54 Antibody
SKU:
CAB15227
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCH4, PCH5, PCH2A, sen54, SEN54L, TSEN54
Positive Sample:
HeLa, MCF7, 293T, Mouse kidney, Rat kidney
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2. RRID AB_2762121 Gene ID 283989 Swiss Prot Synonym PCH4; PCH5; PCH2A; sen54; SEN54L; TSEN54
Purification Method:
Affinity purification
Gene ID:
283989
RRID:
AB_2762121
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TSEN54 Rabbit pAb (CAB15227) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
