The TUBB8 Antibody (CAB12617) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and shows high reactivity with human samples, making it suitable for use in various research applications such as Western blotting.TUBB8 plays a crucial role in cell division and is particularly important for the development and function of cilia and flagella. Mutations in the TUBB8 gene have been linked to a variety of genetic disorders affecting ciliary function, highlighting the importance of studying this protein in the context of ciliopathies and related conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TUBB8 Antibody
SKU:
CAB12617
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OOMD, OOMD2, OZEMA2, bA631M21.2, TUBB8
Positive Sample:
LO2, Jurkat, HepG2, A-549
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
55kDa
The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility.
Purification Method
Affinity purification
Gene ID
347688
RRID
AB_2759461
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TUBB8 Rabbit pAb (CAB12617) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using TUBB8 Rabbit pAb (CAB12617) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
