The UBE2B Antibody (CAB6315) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds specifically to the UBE2B protein, allowing for detection and analysis in a variety of cell types.UBE2B is essential for the proper function of the ubiquitin-proteasome system, which plays a crucial role in regulating protein degradation and turnover. Dysregulation of this system has been implicated in a variety of diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UBE2B Antibody
SKU:
CAB6315
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HR6B, UBC2, HHR6B, RAD6B, E2-17kDa, UBE2B
Positive Sample:
MCF7, SW480, Jurkat, NIH/3T3, Mouse heart, Mouse brain, Mouse skeletal muscle, Rat heart, Rat brain
Cellular Localization:
Cell Membrane, Nucleus.
Calculated MW:
17kDa
Observed MW:
16kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
Purification Method
Affinity purification
Gene ID
7320
RRID
AB_2766920
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UBE2B Rabbit pAb (CAB6315) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
