Description
UBE4B Antibody (CAB17609)
The UBE4B Antibody (CAB17609) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in Western blot applications.UBE4B plays a crucial role in maintaining cellular homeostasis by targeting proteins for degradation through the ubiquitin-proteasome system. Dysregulation of UBE4B has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | UBE4B Antibody |
| SKU: | CAB17609 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | EVDE NDRR EKRS LSDK EPSS GPEV SEEQ ALQL VCKI FRVS WKDR DRDV IFLS SLSA QFKQ NPKE VFSD FKDL IGQI LMEV LMMS TQTR DENP FASL TATS QPIA AAAR SPDR NLLL NTGS N | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | E4, UFD2, HDNB1, UBOX3, UFD2A, UBE4B |
| Positive Sample: | Raji, OVCAR3, HeLa, Mouse testis |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 146kDa |
| Observed MW: | 146kDa |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 10277 |
| RRID | AB_2772785 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using UBE4B Rabbit pAb (CAB17609) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
