The UCKL1 Antibody (CAB15851) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is known for its high specificity and sensitivity in detecting UCKL1 in human samples. It has been validated for use in various applications, including Western blot and immunohistochemistry.UCKL1, also known as Uracil-cytidine kinase-like 1, plays a crucial role in regulating the cell cycle and promoting cell growth and division. Its dysregulation has been linked to various diseases, including cancers and developmental disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UCKL1 Antibody
SKU:
CAB15851
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UCK1L, URKL1, UCKL1
Positive Sample:
Mouse heart, Mouse lung, Rat pancreas
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
61kDa
Observed MW:
61kDa
The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
54963
RRID
AB_2763278
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UCKL1 Rabbit pAb (CAB15851) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using UCKL1 Rabbit pAb (CAB15851) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
