The UFC1 Monoclonal Antibody (CAB21154) is a high-quality antibody developed for reliable detection and analysis of target proteins. UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]). RRID Gene ID 51506 Swiss Prot Synonym NEDSG; HSPC155; UFC1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UFC1 Monoclonal Antibody
SKU:
CAB21154
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3001
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NEDSG, HSPC155, UFC1
Positive Sample:
HepG2, MCF7, THP-1, Mouse pancreas, Rat pancreas
Cellular Localization:
Extracellular Exosome.
Calculated MW:
19kDa
Observed MW:
19kDa
UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]). RRID Gene ID 51506 Swiss Prot Synonym NEDSG; HSPC155; UFC1
Purification Method:
Affinity purification
Gene ID:
51506
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using UFC1 Rabbit mAb (CAB21154) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of various lysates, using UFC1 Rabbit mAb (CAB21154) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
