The UIMC1 Antibody (CAB7244) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed from rabbit serum, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.UIMC1, also known as KIAA0101 or p15(PAF), plays a crucial role in maintaining genomic stability and preventing the accumulation of DNA damage. Dysregulation of UIMC1 has been linked to various cancers, making it a promising target for cancer research and therapy development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UIMC1 Antibody
SKU:
CAB7244
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAP80, X2HRIP110, UIMC1
Positive Sample:
MCF7, 293T, Mouse thymus, Rat brain, HeLa
Cellular Localization:
Nucleus.
Calculated MW:
80kDa
Observed MW:
120kDa/100kDa
This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51720
RRID
AB_2767792
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UIMC1 Rabbit pAb (CAB7244) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from HeLa cells using UIMC1 Rabbit pAb (CAB7244) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 60 s.
