The ULK1 Polyclonal Antibody (CAB21679) is a valuable tool for researchers studying ULK1, a key protein involved in the regulation of autophagy, a cellular process essential for maintaining cell health and survival. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to ULK1, this antibody enables the detection and analysis of ULK1 protein levels in various cell types, making it an ideal choice for autophagy-related studies in the fields of cell biology and disease research.
ULK1, a serine/threonine kinase, plays a crucial role in initiating the autophagy process by phosphorylating downstream targets involved in autophagosome formation. Dysregulation of ULK1 activity has been implicated in a variety of diseases, including neurodegenerative disorders, cancer, and metabolic diseases. By studying ULK1 with the help of this antibody, researchers can gain valuable insights into the mechanisms of autophagy and potentially identify new therapeutic targets for treating autophagy-related diseases.
Product Name:
ULK1 Polyclonal Antibody
SKU:
CAB21679
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant protein of human ULK1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
ATG1; ATG1A; UNC51; hATG1; Unc51.1; ULK1
Positive Sample:
Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Preautophagosomal structure, cytosol.
Calculated MW:
113kDa
Observed MW:
140kDa
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
Purification Method:
Affinity purification
Gene ID:
8408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse thymus, using ULK1 Rabbit pAb (CAB21679) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
