The USO1 Antibody (CAB16079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity in detecting USO1 in human samples, making it a reliable choice for Western blot applications.USO1, a component of the membrane-trafficking system, plays a crucial role in maintaining cellular organization and protein transport. Its involvement in vesicle fusion processes highlights its importance in cellular function and signaling pathways.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
USO1 Antibody
SKU:
CAB16079
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8615
RRID
AB_2763520
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using USO1 Rabbit pAb (CAB16079) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using USO1 Rabbit pAb (CAB16079) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using USO1 Rabbit pAb (CAB16079) at dilution of 1:50. Blue: DAPI for nuclear staining.
