Description
USP28 Antibody (PACO45633)
The USP28 Polyclonal Antibody (PACO45633) is a valuable tool for researchers studying USP28, a protein involved in various cellular processes such as DNA repair, cell cycle regulation, and apoptosis. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting USP28 in human samples, making it suitable for use in Western blot assays.USP28, a deubiquitinase enzyme, plays a crucial role in maintaining protein stability and cellular homeostasis. Dysregulation of USP28 has been implicated in cancer development, making it a potential target for anti-cancer therapies. By using the USP28 Polyclonal Antibody, researchers can investigate the expression levels and localization of USP28 in different cell types, contributing to a better understanding of its functional roles in normal and disease states.
Furthermore, the USP28 Polyclonal Antibody is an essential tool for exploring the molecular mechanisms underlying USP28 activity and its interactions with other proteins. With its reliable performance and versatility in research applications, this antibody is a valuable asset for scientists studying USP28 and its implications in cancer biology and other cellular processes.
| Antibody Name: | USP28 Antibody (PACO45633) |
| Antibody SKU: | PACO45633 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 28 protein (430-580AA) |
| Form: | Liquid |
| Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: USP28 antibody at 3.08µg/ml + Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 123, 120, 67 kDa. Observed band size: 123 kDa.. |
 | Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO45633 at dilution of 1:100. |
| Background: | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells. |
| Synonyms: | Ubiquitin carboxyl-terminal hydrolase 28 (EC 3.4.19.12) (Deubiquitinating enzyme 28) (Ubiquitin thioesterase 28) (Ubiquitin-specific-processing protease 28), USP28, KIAA1515 |
| UniProt Protein Function: | USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Protease; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 11q23.2 Cellular Component: nuclear body; nucleoplasm; protein complex Molecular Function:protein binding; ubiquitin-specific protease activity Biological Process: cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein deubiquitination; Ras protein signal transduction; regulation of protein stability; response to DNA damage stimulus; response to ionizing radiation |
| NCBI Summary: | The protein encoded by this gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of this gene is seen in several cancers. [provided by RefSeq, Oct 2016] |
| UniProt Code: | Q96RU2 |
| NCBI GenInfo Identifier: | 20140700 |
| NCBI Gene ID: | 57646 |
| NCBI Accession: | Q96RU2.1 |
| UniProt Secondary Accession: | Q96RU2,Q6NZX9, Q9P213, B0YJC0, B0YJC1, |
| UniProt Related Accession: | Q96RU2 |
| Molecular Weight: | 122kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 28 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 28 |
| NCBI Official Symbol: | USP28Â Â |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 28 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 28 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28 |
| Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | USP28Â Â |
