The USP53 Polyclonal Antibody (CAB24873) is a high-quality antibody developed for reliable detection and analysis of target proteins. nan
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
USP53 Polyclonal Antibody
SKU:
CAB24873
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
USP53, ubiquitin specific peptidase 53
Positive Sample:
Mouse placenta
Cellular Localization:
Cell Junction, Tight Junction.
Calculated MW:
120kDa
Observed MW:
120kDa
Purification Method
Affinity purification
Gene ID
54532
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse placenta using USP53 Rabbit pAb (CAB24873) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded Human liver cancer using USP53 Rabbit pAb(CAB24873) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
