The VAMP8 Antibody (CAB13915) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the VAMP8 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in cell biology and neuroscience.VAMP8, also known as vesicle-associated membrane protein 8, plays a pivotal role in intracellular membrane fusion events, particularly in the regulation of vesicle trafficking within the cell. Understanding the function and regulation of VAMP8 is essential for unraveling the mechanisms underlying cellular communication, secretion, and synaptic transmission.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VAMP8 Antibody
SKU:
CAB13915
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDB, VAMP-8, VAMP8
Positive Sample:
HeLa, NIH/3T3, PC-12, Mouse kidney, Mouse liver, Rat lung
Cellular Localization:
Cell Membrane, Early Endosome Membrane, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
11kDa
Observed MW:
11kDa
This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.
Purification Method
Affinity purification
Gene ID
8673
RRID
AB_2760768
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VAMP8 Rabbit pAb (CAB13915) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
