The VB6 (Vitamin B6) ELISA Kit is specifically designed for the precise measurement of Vitamin B6 levels in various samples including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Vitamin B6, also known as pyridoxine, is a vital nutrient that plays a crucial role in numerous biological processes such as metabolism, neurotransmitter synthesis, and immune function.
Deficiencies in Vitamin B6 have been linked to various health issues including anemia, cognitive decline, and skin problems, highlighting the importance of monitoring Vitamin B6 levels for overall health and well-being.Whether studying the effects of Vitamin B6 supplementation, investigating its role in certain diseases, or simply monitoring levels for general health assessments, the VB6 ELISA Kit provides researchers with a reliable and efficient tool for accurate Vitamin B6 detection.
Product Name:
VB6 (Vitamin B6) ELISA Kit
SKU:
UNES00005
Target:
VB6 (Vitamin B6) (UNES00005)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VB6. During the reaction, VB6 in the sample or standard competes with a fixed amount of VB6 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VB6. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB6 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
93-104
90-101
Average (%)
94
99
95
1:4
Range (%)
87-100
89-100
86-101
Average (%)
92
95
93
1:8
Range (%)
87-98
94-109
86-103
Average (%)
93
101
94
1:16
Range (%)
89-103
94-107
90-103
Average (%)
95
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.75
14.63
46.67
4.5
13.37
51.21
Standard deviation
0.25
0.63
2.41
0.3
0.68
2.02
C V (%)
5.26
4.31
5.16
6.67
5.09
3.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VB6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes VB6 in samples. No significant cross-reactivity or interference between VB6 and analogues was observed.
