The VD3 (Vitamin D3) ELISA Kit is specifically designed for the precise measurement of Vitamin D3 levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for research purposes.Vitamin D3, also known as cholecalciferol, plays a crucial role in regulating calcium and phosphorus levels in the body, promoting bone health, immune function, and overall well-being. Deficiencies in Vitamin D3 have been linked to a variety of health conditions, including osteoporosis, autoimmune diseases, and cardiovascular disorders, highlighting the importance of monitoring Vitamin D3 levels.
By utilizing the VD3 ELISA Kit, researchers can gain valuable insights into Vitamin D3 status, assess potential deficiencies, and explore the impact of Vitamin D3 on various physiological processes. This kit provides a reliable and efficient way to measure Vitamin D3 levels, enabling researchers to advance their understanding of Vitamin D3 biology and its implications for human health.
Product Name:
VD3 (Vitamin D3) ELISA Kit
SKU:
UNES00010
Target:
VD3 (Vitamin D3) (UNES00010)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD3. During the reaction, VD3 in the sample or standard competes with a fixed amount of VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
86-97
89-102
Average (%)
100
92
94
1:4
Range (%)
93-108
86-102
86-100
Average (%)
101
93
93
1:8
Range (%)
94-109
85-100
89-103
Average (%)
101
92
94
1:16
Range (%)
96-109
84-96
87-102
Average (%)
101
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
85-97
92
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.75
12.74
45.67
4.34
12.36
44.41
Standard deviation
0.29
0.63
2.42
0.27
0.67
2.15
C V (%)
6.11
4.95
5.3
6.22
5.42
4.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VD3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes VD3 in samples. No significant cross-reactivity or interference between VD3 and analogues was observed.
