The VPRBP Polyclonal Antibody (CAB24518) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in Western blot applications, providing reliable detection and analysis of the VPRBP protein in human samples.VPRBP, also known as DCAF1, is a key component of the VPRBP-DDB1-CUL4 E3 ubiquitin ligase complex, which plays a crucial role in targeting specific proteins for degradation. Dysregulation of this complex has been linked to various diseases including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
VPRBP Polyclonal Antibody
SKU:
CAB24518
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIP, VPRBP
Positive Sample:
Neuro-2a, Mouse testis
Calculated MW:
118kDa/168kDa/169kDa
Observed MW:
170kDa
Enables estrogen receptor binding activity and histone kinase activity (H2A-T120 specific). Involved in cell competition in a multicellular organism; histone H2A-T120 phosphorylation; and negative regulation of transcription by RNA polymerase II. Located in fibrillar center and nucleoplasm. Part of Cul4-RING E3 ubiquitin ligase complex. Colocalizes with COP9 signalosome.
Purification Method
Affinity purification
Gene ID
9730
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using VPRBP Rabbit pAb (CAB24518) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
