The VRK1 Antibody (CAB7745) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the VRK1 protein, this antibody enables researchers to study the function and expression of VRK1 in a variety of cell types.VRK1 is a serine/threonine kinase that is involved in various cellular processes, including cell division, DNA repair, and apoptosis. Dysregulation of VRK1 has been implicated in diseases such as cancer and neurodegenerative disorders, making it an important target for therapeutic intervention.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VRK1 Antibody
SKU:
CAB7745
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCH1, PCH1A, K1
Positive Sample:
293T, MCF-7, Jurkat, A-549, HeLa
Cellular Localization:
Cytoplasm, Nucleus, Cytoskeleton, Spindle.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Purification Method
Affinity purification
Gene ID
7443
RRID
AB_2772874
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] VRK1 Rabbit pAb (CAB7745) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of lysates from wild type(WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit pAb (CAB7745) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using [KO Validated] VRK1 Rabbit pAb (CAB7745) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
