The VTI1B Antibody (CAB7062) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the VTI1B protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and molecular biology.VTI1B is a key player in the regulation of vesicle trafficking and fusion processes within the cell, contributing to cellular homeostasis and function. Its involvement in intracellular transport pathways makes it a target of interest for investigations into diseases related to protein trafficking abnormalities, such as neurodegenerative disorders and metabolic diseases.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
VTI1B Antibody
SKU:
CAB7062
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasmic Granule, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
27kDa
Observed MW:
27kDa
Enables SNARE binding activity and chloride channel inhibitor activity. Involved in regulation of protein localization to plasma membrane. Located in several cellular components, including endosome membrane; lysosomal membrane; and perinuclear region of cytoplasm.
Purification Method
Affinity purification
Gene ID
10490
RRID
AB_2767617
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse brain, using VTI1B Rabbit pAb (CAB7062) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using VTI1B Rabbit pAb (CAB7062) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.