The WDR45 Antibody (CAB7581) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the WDR45 protein, this antibody allows for the detection and analysis of WDR45 in various cell types, making it ideal for studies in neurobiology and related fields.WDR45, also known as WIPI4, plays a crucial role in the autophagy pathway by regulating the formation of autophagosomes. Mutations in the WDR45 gene have been associated with neurodegenerative disorders such as beta-propeller protein-associated neurodegeneration (BPAN).
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
WDR45 Antibody
SKU:
CAB7581
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JM5, NBIA4, NBIA5, WDRX1, WIPI4, WIPI-4, WDR45
Positive Sample:
Mouse skeletal muscle, Rat lung
Cellular Localization:
Cytosol.
Calculated MW:
40kDa
Observed MW:
39kDa/35kDa
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.
Purification Method
Affinity purification
Gene ID
11152
RRID
AB_2768103
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using WDR45 Rabbit pAb (CAB7581) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of NIH/3T3 cells using WDR45 Rabbit pAb (CAB7581) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
