The Xanthine Oxidase (XDH) Monoclonal Antibody (CAB9022) is a high-quality antibody developed for reliable detection and analysis of target proteins. Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Xanthine Oxidase (XDH) Monoclonal Antibody
SKU:
CAB9022
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1385
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:2000
IF/ICC
1:200 - 1:400
IF-P
1:200 - 1:400
IHC-P
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XO, XOR, XAN1, Xanthine Oxidase (XDH)
Positive Sample:
Hep G2, Rat lung
Cellular Localization:
Cytoplasm, Peroxisome, Secreted.
Calculated MW:
146kDa
Observed MW:
150kDa
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Purification Method
Affinity purification
Gene ID
7498
RRID
AB_2863643
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Xanthine Oxidase (XDH) Rabbit mAb (CAB9022) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Confocal imaging of NIH/3T3 cells using Xanthine Oxidase (XDH) Rabbit mAb (CAB9022, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Xanthine Oxidase (XDH) Rabbit mAb (CAB9022) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Xanthine Oxidase (XDH) Rabbit mAb (CAB9022) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
