The XRCC1 Monoclonal Antibody (CAB4135) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting XRCC1 in human samples, making it an excellent choice for Western blot applications.XRCC1 is essential for repairing single-strand breaks in DNA and plays a crucial role in maintaining genomic stability. Dysregulation of XRCC1 has been linked to an increased risk of cancer and other genetic disorders, making it a significant target for research in the fields of oncology and genomics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
XRCC1 Monoclonal Antibody
SKU:
CAB4135
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0915
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RCC, SCAR26, XRCC1
Positive Sample:
K-562, Mouse testis, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
69kDa
Observed MW:
82kDa
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Purification Method
Affinity purification
Gene ID
7515
RRID
AB_2863192
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using XRCC1 Rabbit mAb (CAB4135) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
