The Zinc-alpha2-glycoprotein (ZAG/AZGP1) Monoclonal Antibody (CAB11680) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody is specifically designed for use in Western blot applications, providing reliable and accurate detection of ZA2G/AZGP1 in human samples.By targeting the ZA2G/AZGP1 protein, this antibody allows for detailed analysis and understanding of its function in different cell types. With its high reactivity and specificity, researchers can confidently utilize this antibody in immunology and cancer research studies.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZAG, ZA2G, Zinc-α2-glycoprotein (ZAG/AZGP1)
Positive Sample:
Hep G2
Cellular Localization:
Secreted.
Calculated MW:
34kDa
Observed MW:
35-45kDa
Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space.
Purification Method
Affinity purification
Gene ID
563
RRID
AB_2861627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (CAB11680) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (CAB11680) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
