The ZCCHC11 Antibody (CAB5972) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been rigorously validated for Western blot applications. By specifically targeting the ZCCHC11 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component for studies in RNA biology and gene regulation.ZCCHC11 plays a crucial role in RNA metabolism, including mRNA modification and processing, making it an attractive target for investigating diseases related to RNA dysregulation such as cancer, neurodegenerative disorders, and viral infections.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZCCHC11 Antibody
SKU:
CAB5972
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAPD3, TENT3A, ZCCHC11
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
185kDa
Observed MW:
185kDa
Enables RNA uridylyltransferase activity. Involved in RNA metabolic process; negative regulation of transposition, RNA-mediated; and stem cell population maintenance. Located in cytoplasmic ribonucleoprotein granule; cytosol; and nucleolus. Implicated in liver benign neoplasm. Biomarker of breast cancer.
Purification Method
Affinity purification
Gene ID
23318
RRID
AB_2766692
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using ZCCHC11 Rabbit pAb (CAB5972) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of U2OS cells using ZCCHC11 Rabbit pAb (CAB5972) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using ZCCHC11 Rabbit pAb (CAB5972) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
