The ZDHHC20 Antibody (CAB17982) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein-cysteine S-palmitoyltransferase activity and zinc ion binding activity. Involved in peptidyl-L-cysteine S-palmitoylation. Located in intracellular membrane-bounded organelle and plasma membrane. Is integral component of Golgi membrane.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZDHHC20 Antibody
SKU:
CAB17982
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DHHC20, DHHC-20, 4933421L13Rik, ZDHHC20
Positive Sample:
HeLa, MCF7, SKOV3, A-549, Mouse brain, Mouse lung, Mouse testis, Rat brain, Rat lung
Cellular Localization:
Endoplasmic Reticulum, Golgi Apparatus, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
42kDa
Observed MW:
42kDa
Enables protein-cysteine S-palmitoyltransferase activity and zinc ion binding activity. Involved in peptidyl-L-cysteine S-palmitoylation. Located in intracellular membrane-bounded organelle and plasma membrane. Is integral component of Golgi membrane.
Purification Method
Affinity purification
Gene ID
253832
RRID
AB_2861783
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZDHHC20 Rabbit pAb (CAB17982) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
