The ZEB2 Antibody (CAB5705) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications.ZEB2 is a critical player in the process of EMT, which is implicated in promoting cancer metastasis by allowing tumor cells to become more invasive and migratory. By targeting ZEB2 with this antibody, researchers can better understand its role in driving cancer progression and potentially identify new therapeutic strategies for combating metastatic disease.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZEB2 Antibody
SKU:
CAB5705
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIP1, SIP-1, ZFHX1B, HSPC082, SMADIP1, ZEB2
Positive Sample:
Mouse liver, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
136kDa
Observed MW:
210kDa
The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
9839
RRID
AB_2766464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ZEB2 Rabbit pAb (CAB5705) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using ZEB2 Rabbit pAb (CAB5705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
