The ZFPM1 Antibody (CAB18183) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. ZFPM1 is a zinc finger protein that regulates gene expression during development, particularly in the formation of blood cells and the heart. Dysregulation of ZFPM1 has been linked to various diseases, including certain types of leukemia and congenital heart defects.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZFPM1 Antibody
SKU:
CAB18183
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FOG, FOG1, ZNF408, ZNF89A, ZC2HC11A, ZFPM1
Positive Sample:
K-562, HEL, Mouse spleen
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
105kDa
Observed MW:
105kDa
Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and transcription corepressor activity. Involved in platelet formation; regulation of definitive erythrocyte differentiation; and regulation of gene expression. Part of transcription repressor complex.
Purification Method
Affinity purification
Gene ID
161882
RRID
AB_2861962
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ZFPM1 Rabbit pAb (CAB18183) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using ZFPM1 Rabbit pAb (CAB18183) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
