The A-CoA (Acetyl Coenzyme A) ELISA Kit is designed for the quantification of Acetyl Coenzyme A levels in various biological samples. Acetyl Coenzyme A plays a central role in the metabolism of carbohydrates and fats, acting as a key intermediate in numerous biosynthetic reactions. It is crucial for producing energy in cells, making it a critical component in metabolic functioning. Accurately quantifying levels of Acetyl Coenzyme A is essential for understanding its role in metabolic disorders and for developing targeted therapeutics. Assay Genie's A-CoA ELISA Kit offers unbeatable sensitivity and specificity, believing in the provision of reliable and reproducible results. Made under strict quality control standards, this kit delivers robust performance and is user-friendly, being an optimal choice in both research and clinical settings. Trust Assay Genie's A-CoA ELISA Kit for precise and reliable quantitation of this key metabolic biomarker in your experiments.
Product Name:
A-CoA (Acetyl Coenzyme A) ELISA Kit
SKU:
AEES00006
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
