The GABA transaminase (ABAT) Antibody (CAB5299) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through rabbit immunization, has a high affinity for human samples and has been tested for efficacy in Western blot applications. By binding specifically to the ABAT protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations related to neuroscience and neurological disorders.ABAT is a key enzyme in the catabolism of GABA, an important neurotransmitter in the central nervous system.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GABA transaminase (ABAT) Antibody
SKU:
CAB5299
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GABAT, NPD009, GABA-AT, GABA transaminase (ABAT)
Positive Sample:
HepG2, Mouse kidney, Rat kidney
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
56kDa
Observed MW:
50kDa
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method
Affinity purification
Gene ID
18
RRID
AB_2766111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GABA transaminase (ABAT) Rabbit pAb (CAB5299) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded mouse brain using GABA transaminase (GABA transaminase (ABAT)) Rabbit pAb (CAB5299) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
